detection of genetically modified maize in processed foods sold commercially in iran by qualitative pcr

Authors

maryam rabiei 1- food and drug laboratory research center, food and drug organization, ministry of health and medical education, tehran, iran. 2-department of molecular biology, food and drug control reference laboratory, ministry of health and medical education, tehran, iran.

mehrangiz mehdizadeh department of molecular biology, food and drug control reference laboratory, ministry of health and medical education, tehran, iran.

hossein vahidi department of biotechnology, faculty of pharmacy, shahid beheshti university of medical sciences and health services, tehran, iran.

mahmoud alebouyeh 1- food and drug laboratory research center, food and drug organization, ministry of health and medical education, tehran, iran. 2- department of molecular biology, food and drug control reference laboratory, ministry of health and medical education, tehran, iran.

abstract

detection of genetically modified organisms (gmos) in food is an important issue for all the subjects involved in food control and customer’s right. due to the increasing number of gmos imported to iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. in this study, following the extraction of genomic dna from processed foods sold commercially in iran, qualitative pcr was performed to detect genetically modified maize. the recombinant dna target sequences were detected with primers highly specific for each investigated transgene such as camv35s gene, bt-11, mon810 and bt-176 separately. based on the gel electrophoresis results, bt-11 and mon810 events were detected in some maize samples, while, in none of them bt-176 modified gene was detected. for the first time, the results demonstrate the presence of genetically modified maize in iranian food products, reinforcing the need for the development of labeling system and valid quantitative methods in routine analyses.

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Journal title:
the iranian journal of pharmaceutical research

جلد ۱۲، شماره ۱، صفحات ۲۵-۳۰

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